ZFIN Image: Noschka et al., 2021, Figure 3

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Figure Caption

Figure 3

Effect of Angiogenin on macrophage viability and Mtb multiplication. (A) Macrophages were incubated for 24 h with Angiogenin (10 μM, PSL Heidelberg) or diluent. Macrophage viability was quantified using a PrestoBlue™ assay and is given in % of the un-treated sample. Bars represent the mean viability of macrophages (%) ± SD calculated from triplicates of three independent experiments. (B) Macrophages were infected with Mtb, followed by incubation with Angiogenin (10 μM) for 4 days. Lysates were plated on 7H11 agar plates and the number of CFU counted after 21 days of incubation. Mtb-multiplication was calculated by comparison of the CFU determined at d4 and d0. The graph gives the mean values ± SD of n-fold mycobacterial growth as compared to d0 for six independent donors. Statistical analysis was performed using a paired t-test.

Acknowledgments

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