Fig. 5

Fig. 5 Fig. 5. Disruption of Ras signaling impairs lamellipodia-like protrusion formation. (A, B) Lateral views of mosaic embryo carrying the Tg (hsp70:dN-Ras); TgBAC(cxcr4b:LifeAct-Citrine) cells. Embryos were heat shocked at 28 hpf, allowed to recover for 1h, and then mounted for live imaging (Movie 7). (A?,B?) 2X magnified images of boxed cells. Note abnormal spikey lamellipodia-like protrusions. (C) Percentage of time a cell contained a lamellipodia-like protrusion comparing early (1.5?2.5 ?h) post heat shock to late post heat shock (5?6 ?h) stages (n ?= ?12 ?cells from 4 embryos). (D and E) Average lamellipodia-like protrusion length and size per cell comparing early (1.5?2.5 ?h) to late post heat shock (5?6 ?h) stages (n ?= ?9 ?cells from 4 embryos). Asterisks ?= ?lamellipodia-like protrusions. Scale bar ?= ?10 ??m.