Fig. 2

Fig. 2 Fig. 2. Arp2/3 activity is required for brush-like protrusion formation and pLLP migration. (A) Effects of increasing concentration of CK-666 on pLLP migration in comparison to vehicle and inactive control drug, CK-689. (B) Mosaically labeled embryos carrying TgBAC(cxcr4b:LifeAct-Citrine) transgene were first exposed to DMSO (vehicle) and imaged for 1.5 ?h beginning at 30 hpf (Movie 2). (C) Following DMSO treatment, embryos were then exposed to the Arp2/3 inhibitor CK-666 for 4 ?h and concurrently imaged (Movie 2). Note the loss of brush-like protrusions following the treatment. Images show lateral views. (B?,C?) 1.8X zoom of boxed cells in B and C. (D) Percentage of time a given cell contained a brush-like protrusion during vehicle and the inhibitor treatment (n ?= ?11 ?cells from 6 embryos). (E) Migration speed of the whole pLLP during vehicle and inhibitor treatment (n ?= ?8 whole pLLPs). Asterisks ?= ?brush-like protrusions. Scale bar ?= ?10 ??m.