ZFIN Image: Kula-Alwar et al., 2020, Fig. 4

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Figure Caption

Fig. 4

Mef2ca^+/⁻;mef2cb^−/−mutant embryos show reduced ventricular size compared to siblings. (A) Confocal images of 3 dpf (top panels) or 5 dpf hearts (bottom panels) from a cross of mef2ca^+/⁻;mef2cb^+/⁻;Tg(myl7:EGFP) to either mef2ca^+/+;mef2cb^+/⁻;Tg(myl7:EGFP) or mef2ca^+/⁻;mef2cb^+/+;Tg(myl7:EGFP) fish. mef2ca^+/⁻;mef2cb^−/⁻ embryos have a smaller heart at 3 and 5 dpf. Scale bars = 50 μm. (B,C) Volume, trabecular length and width were quantified for embryos from crosses in A designed to test Mef2ca (B) and Mef2cb (C) function. One-way ANOVA analysis followed by Bonferroni post hoc tests, p-values indicated on graphs. Graphs show mean ± S.E.M. N for each group shown on bars.

Acknowledgments

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Reprinted from Developmental Biology, 470, Kula-Alwar, D., Marber, M.S., Hughes, S.M., Hinits, Y., Mef2c factors are required for early but not late addition of cardiomyocytes to the ventricle, 95-107, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.