Fig. 5 Smpx localizes to the ciliated cells of the zebrafish pronephros. (A) Cross-section of the trunk of a 48 hpf embryo; the pronephric ducts are labeled in green with the antibody against Smpx. (A′) 5X magnifications of the area enclosed in the rectangle in A; Smpx labels the apical membrane of the ciliated cells facing the lumen of the duct, as also confirmed (B) by the co-labeling with antibodies against Smpx and acetylated tubulin, where the lumen is populated exclusively by the acetylated tubulin-based kinocilia (red) sprouting from the apical membrane and devoid of Smpx (green). Images are all confocal Z-stacks taken from paraffin sections. pd, pronephric duct; n, notochord; nt, neural tube. Scale bars = 50 μm in A; 20 μm in C.
Reprinted from Gene expression patterns : GEP, 36, Ghilardi, A., Diana, A., Prosperi, L., Del Giacco, L., Expression pattern of the small muscle protein, X-linked (smpx) gene during zebrafish embryonic and larval developmental stages, 119110, Copyright (2020) with permission from Elsevier. Full text @ Gene Expr. Patterns