Fig. 5

Fig. 5

Effect of deletion of C2cd4b on sex hormones and hormone release from the pituitary gland. (a) RT-qPCR on samples from the pituitary glands shows basal level of mRNA expression of C2cd4b in animals deleted for this gene compared with WT mice. (b, c) Measurement of E2 and testosterone levels upon deletion of C2cd4b in female and male mice maintained on either an RC diet or an HFD. (d, e) Circulating LH levels in C2cd4b-null and WT female (d) and male (e) mice after injections with saline (154 mmol/l NaCl; vehicle), E2 or testosterone (animals were maintained on an RC diet). (f, g) Circulating FSH levels in C2cd4b-null and WT female (f) and male (g) mice after injections with saline (vehicle), E2 or testosterone (animals were maintained on an RC diet). Female (F)^+/+n=6, F^−/−n=5; male (M)^+/+n=4; M^−/−n=5. **p<0.01, ***p<0.001, unpaired Student’s t test. Values represent mean ± SEM