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Figure 1 Construction of a transgenic vector. (A) Plasmid map of the transgenic vector containing a krt8 promoter, grass carp interferon (IFN1) cDNA and His-tag. (B) The expression of EGFP under fluorescence microscope. krt8-1dpf: One day after fertilization; krt8-5dpf: five days after fertilization. (C) Western blot analysis of IFN1 in 293T cells. Group pcDNA-IFN1-His: 293T cells were transfected with pcDNA-IFN1-His; pT2-krt8-IFN1-1, 2 and 3: 293T cells were transfected with pT2-krt8-IFN1; Group pcDNA3.1-His: 293T cells were transfected with pCDNA3.1-His.
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