ZFIN Image: Li et al., 2020, FIGURE 2

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Figure Caption

FIGURE 2

Nar protects against gentamicin ototoxicity. (A) Animals were fixed and immunostained for MyosinVIIa (green) and DAPI. 5 dpf zebrafish were incubated with 0 μmol/L to 150 μmol/L GM for 3 h or pre‐treated with 0 µmol/L to 400 µmol/L of Nar for 3 h and then co‐treated with Nar and GM (100 µmol/L) for 3 h. (B) Diagram of the assay for A. (C and D) Quantification of the number of hair cells (MyosinVIIa⁺) per neuromast after the different treatments represented as mean ± SEM (n = 10). Control animals were exposed to vehicle alone (DMSO). **P < 0.01, ***P < 0.001. Black asterisks compared to DMSO‐treated animals. Red asterisks compared versus the corresponding GM concentration. (E) Scores for neuromast morphology of each group (see Materials and Methods). Scale bar equals 5μm

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