FIGURE 2

FIGURE 2

Carbofuran exacerbated the spns1 deficiency phenotype and shortened the survival of spns1 mutant zebrafish. A, Sorts of yolk opaqueness phenotypes in spsn1^−/− (homozygous) zebrafish based on the extent of opacity in the yolk and/or yolk extension: partially opaque (yellow colour) and mostly opaque (red colour). Wild (spns1^+/+) and heterozygous (spns1^+/−) zebrafish do not show such opaqueness (dark blue colour). B, Effect of carbofuran on the opaqueness phenotype of spns1 mutant fish and death record. The opaqueness phenotype and death records were not affected by 10 μmol/L carbofuran exposure. However, the mostly opaque phenotype and death of embryos of 100 μmol/L carbofuran treated group were found at earlier times than fishes of the control group (egg water treatment). C, Survival curves of spns1 mutant fishes for carbofuran treatment at 10 and 100 μmol/L concentrations. By 100 μmol/L carbofuran exposure, the survival of spsn1 mutant fishes was significantly declined (P = .0003)