Fig. 3

Fig. 3

DNMT3a modulates thermal performance curves. A significant interaction between genotype and developmental temperature manifests as a progressive decline in swimming performance (U_crit) (a) of warm-developed fish (red symbols and lines) from control to DNMT3aa^−/− and to DNMT3aa^−/−ab^−/− where U_crit was lowest. The genotype x developmental temperature interaction determining citrate synthase (CS) activity (b) results from higher activity in warm-developed DNMT3aa^−/− fish compared to cold-developed fish. As for U_crit, activity of warm-developed DNMT3aa^−/−ab^−/− fish was lowest but similar to control. Marginal means (± s.e.) are shown across both acclimation treatments in a and b. Sample sizes were n = 12 for U_crit (24 for marginal means shown) and n = 8–9 for CS activity (16–18 for marginal means). Developmental temperature interacted with acclimation temperature to determine U_crit (c), and CS activity (d); marginal means (± s.e.) across all genotypes and test temperatures are shown