FIGURE 6

FIGURE 6

Naringin promotes the regeneration of functional hair cells. A, Still imaging of hair cells of zebrafish larvae 3, 4 and 5 d after FM1‐43 labelled (green) fertilization. B, Scatter plot of FM1‐43 labelled hair cell count at each time‐point (***P < 0.001; mean ± SEM; n = 10). Black asterisks correspond to 3 dpf animals, red asterisks correspond to 4 dpf animals. Hair cells with mechanotransduction channel activity (FM1‐43⁺, green) are defined as functional and mature hair cells. (C) Still imaging of hair cells after different treatment: 5 dpf Wild‐type larvae were incubated with vehicle alone for 24 h after being treated with vehicle or serial dilutions of Ototoxic drugs (Control group), or with Nar 400 µmol/L prevention and co‐incubation treatment (Co‐Nar recovery group); larvae were treated by ototoxic drugs and then incubated with Nar for 24 h (Nar recovery group); 5 dpf zebrafish were incubated with Nar for 6 h or 24 h as the corresponding control. Scale bar equals 5 µm. (D) Diagram of the assay for C. (E) The FM1‐43 fluorescent intensity per neuromast was calculated for each treatment and represented as mean ± SEM. No significant differences were observed between control and treated animals (unpaired Student's t test). (F) Quantification of the number of functional hair cells per neuromast after the different treatments represented as mean ± SEM. ***P < 0.001. Black asterisks compared versus control. Red asterisks compared versus the corresponding Nar concentration