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Figure 3

ID: ZDB-IMAGE-210123-24
Publication: Almeida et al., 2021 - Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration
All Figures: Figures for Almeida et al., 2021

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Figure 3

Transgenic ascl1b-2A-Cre, olig2-2A-Cre and neurod1-2A-Cre lines express functional Cre that promotes recombination at loxP sites in the expected neural progenitor populations. (a) F0 adults harboring precise integration alleles were mated to the recombination reporter line ubi:Switch to generate double transgenic 2A-Cre driver; ubi:Switch embryos. (b–d) Confocal imaging of 3 dpf double transgenic ascl1b-2A-Cre; ubi:switch (b), olig2b-2A-Cre; ubi:switch (c), and neurod1-2A-Cre; ubi:switch (d) larvae shows a switch from GFP to mCherry expression in neural cells derived from ascl1b, olig2, and neurod1 progenitors. (e) A switch from GFP to mCherry expression isn’t detected in single transgenic ubi:Switch larvae. fb forebrain, γ-cry gamma crystallin promoter, EGFP green fluorescent protein, hb hindbrain, mb midbrain, nt neural tube, op olfactory placode, r retina. Scale bar 200 μm.

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