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Figure 1—figure supplement 1.

ID: ZDB-IMAGE-210113-56
Publication: Kroll et al., 2021 - A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
All Figures: Figures for Kroll et al., 2021

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Figure Caption

Figure 1—figure supplement 1. Cas9 and gRNA achieve highest phenotypic penetrance at 1-to-1 ratio.

(A–B) (top) Phenotypic penetrance as gradually more Cas9 was injected; 1:6: 4.75 fmol Cas9, 1:3: 9.5 fmol Cas9, 1:2: 14.25 fmol Cas9, 1:1: 28.5 fmol Cas9. gRNA was kept constant at 28.5 fmol. Pictures of the eye at 2 dpf are examples of the scoring method (reproduced from Figure 1C,D). (bottom) Unviability as percentage of 1-dpf embryos.

Acknowledgments

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