ZFIN Image: Mignani et al., 2020, FIGURE 2

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Figure Caption

FIGURE 2

Morphological analysis of embryos micro-injected with an ATG-blocking morpholino. Embryos were injected with the gene a-specific (ATG) or the control (STD) morpholino at 1/2 cell stage and analyzed at the microscope at 48 and 72 hpf. (A) Representative images showing the main features of controls and morphants at 48 hpf. Numbers in each panel represent total embryos used for the experiment and embryos with the phenotype shown in the image. (B) The graph shows the percentage distribution of embryos with normal or abnormal (mild and severe) morphology in each microinjection category. (C) The graph show the mean size of the eye diameter in controls and morphants (N > 40) at 48 and 72 hpf. Size bar = 500 μm. ****P < 0.0001 (unpaired, two-tailed T-Test). Experiments were repeated at least three times.

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Acknowledgments

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