ZFIN Image: Wague et al., 2020, Figure 4.

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Figure Caption

Figure 4. Cysteine modification at the G182 position leads to TREK1 activation.

(A) Representative time courses of the response of TREK1 wildtype (WT) or TREK1 G182C channels to treatment with 2 mM MMTS. Shown is the recorded current at 0 mV holding potential, measured every 5 s for 5 min, with current values normalized to the initial value recorded at the beginning of the time course (B) Quantification of effect size at the end of the time course (300 s), for TREK1 WT and TREK1 Cys-, a mutant TREK1 channel lacking all five of the endogenous TREK1 cysteine residues, and for G182 mutants in both of the above backgrounds. Number of replicate experiments indicated. Error bars are mean ± SEM. Statistically significance determined by one-way ANOVA combined with a Sidak multiple comparison test of TREK1 WT versus TREK1 G182C and TREK1 Cys- versus TREK1 cys- G182C. Results indicated, ****p<0.0005. (C) Crystallographically defined structural models of TREK2 in the TM4 up (PDB ID 4xdl, yellow) and TM4 down (PDB ID 4bw5, tan) conformations, highlighting the TM2, TM3, and TM4 helices from only a single subunit. The position of G182 (red sphere) is noted.

Acknowledgments

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