Figure 8

Figure 8

Effects of PBA on the sleeping pattern of MPP+ treated zebrafish larvae. Larvae in the absence (Control) or presence of 500 μM (MPP+) was treated with vehicle (black bars) or 100 μM PBA (white bars). Sleeping pattern were evaluated for (A) sleep fragmentation; (B) sleep ratio; (C) sleep latency (s); (D) swim velocity (mm/s); (E) wake bout duration (s) and (F) sleep bout duration (s). Data are represented as means ± SEM from a single experiment, control group n = 32, MPP+ group n = 31, PBA group n = 32, MPP+/PBA group n = 32. Normality was tested using D’Agostino and Pearson omnibus normality test followed by two-way ANOVA and Tukey’s post hoc analysis. P-value for interaction between neurotoxin exposure and drug treatment is shown above the graph. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, for multiple comparisons.