Figure 6

Figure 6

Effects of GDNF on the sleeping pattern of MPP+-treated zebrafish larvae. Larvae were incubated in the absence (black bars) or presence (white and gray bars) of 500 μM MPP+ and injected with 72.9 ng GDNF (gray bars) at 4 dpf before assessing (A) sleep fragmentation; (B) sleep ratio; (C) sleep latency (s); (D) swim velocity (mm/s); (E) wake bout duration (s) and (F) sleep bout duration (s) at 6 dpf. Data are presented as means ± SEM from a single experiment, control group n = 32, MPP+ group n = 31, GDNF group n = 32. Normality was tested using D’Agostino and Pearson omnibus normality test followed by the two-tailed Kruskal–Wallis test (p-value shown above graph) and Dunn’s post hoc analysis. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, for multiple comparisons.