ZFIN Image: Narumi et al., 2020, FIGURE 4

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Figure Caption

FIGURE 4

Restoration of chemical-induced cleft palate by Wnt agonists. (A) Experimental time course. m.c.: medium change. (B–K) Fluorescence images of the palate at 96 hpf. (B,C) WAF (30 μM) exposure induced cleft palate. (D–F) The cleft palate was rescued by combinatorial treatment with BIO (100 nM), CHIR99021 (300 nM) or WAY-262611 (250 nM). (G,H) VPA (20 μM) exposure induced cleft palate. (I–K) The cleft palate caused by VPA was rescued by combinatorial treatment with BIO (100 nM), CHIR99021 (300 nM) or WAY-262611 (250 nM). White indicates nuclei stained with DAPI. Yellow dotted lines trace the shape of the anterior edge of the plate. (L,M) Frequency of rescued cleft palate. n = 18 (Control), 11 (WAF), 12 (WAF + BIO), 9 (WAF + CHIR), 10 (WAF + WAY) in (L), n = 19 (Control), 18 (VPA), 15 (VPA + BIO), 18 (VPA + CHIR),15 (VPA + WAY) in (M). Scale bar: 50 μm.

Acknowledgments

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