FIGURE 2

FIGURE 2

Chemical-induced cleft palate was induced by inhibition of the canonical Wnt signaling pathway. (A) Experimental time-course. m.c.: medium change. (B–H) Fluorescence images of the palate at 96 hpf. Nuclei of cartilage cells were stained with DAPI. Yellow dotted line indicates the anterior edge of the palate. (I,J) Frequency of palate morphology. n = 20 for each sample. (K,L) Quantification of relative levels of tcf7l1a(K) and lef1(L) mRNA isolated from the neurocranium at 72 and 96 hpf. Each mRNA level was normalized by gapdh mRNA by the comparative CT (2^{– ΔΔCT}) method. Data are shown as mean ± SD from triplicate experiments. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Welch’s t-tests). Scale bar: 50 μm.