Figure 9

Figure 9

Experimental plan showing the timeline of heat-shock treatments and effects of thermal shock on p.R239C aggregates. (a) At 24 hpf, embryos were incubated for 1 h at 37 °C and then recovered at 28 °C. Fluorescence microscope analysis was performed after 3 (t3) and 24 h (t24) from the end of the 1 h heat shock (t1). In Figure (A–H), mutant embryos not shocked (unshocked) are presented at 24 hpf, showing diffuse aggregate formation before and after 3 h. Figure (I–L) show GFAP p.R239C aggregates in embryos at 24 hpf, while, in figure (M–P), the thermal shock effect is shown after 3 h on GFAP p.R239C aggregates. Magnification: 4× (A,E,J,M); 10× (B,C,F,G,J,K,N,O); 20× (D,H,L,P).