IMAGE

Figure 9

ID: ZDB-IMAGE-201229-88
Publication: Candiani et al., 2020 - Alexander Disease Modeling in Zebrafish: An In Vivo System Suitable to Perform Drug Screening
All Figures: Figures for Candiani et al., 2020

Image

Figure Caption

Figure 9

Experimental plan showing the timeline of heat-shock treatments and effects of thermal shock on p.R239C aggregates. (a) At 24 hpf, embryos were incubated for 1 h at 37 °C and then recovered at 28 °C. Fluorescence microscope analysis was performed after 3 (t3) and 24 h (t24) from the end of the 1 h heat shock (t1). In Figure (A–H), mutant embryos not shocked (unshocked) are presented at 24 hpf, showing diffuse aggregate formation before and after 3 h. Figure (I–L) show GFAP p.R239C aggregates in embryos at 24 hpf, while, in figure (M–P), the thermal shock effect is shown after 3 h on GFAP p.R239C aggregates. Magnification: 4× (A,E,J,M); 10× (B,C,F,G,J,K,N,O); 20× (D,H,L,P).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Genes (Basel)