Figure 7

Figure 7 Human EIF2B2 gene rescues zebrafish eif2b2^{sa17223/sa17223} mutants. (A) 11.1 kb zebrafish eif2b2 gene structure. (B) 9.5 kb human EIF2B2 gene structure. (C) Conservation of amino acid sequence between zebrafish eif2b2 and human EIF2B2. (D) Schematic of rescue construct containing Tol2, β-actin, human EIF2B2, and eGFP. (E) Genotype results (at adult age), of an eif2b2^sa17223/+ heterozygous incross, and an eif2b2^sa17223/+ heterozygous zebrafish crossed with two different transgenic alleles (#3 and #4): eif2b2^sa17223/+;Tg3(β-actin:EIF2B2:2A:eGFP) or eif2b2^sa17223/+;Tg4(β-actin:EIF2B2:2A:eGFP) heterozygous zebrafish. (F) Bright-field and immunofluorescent images of eif2b2^{sa17223/sa17223};β-actin:EIF2B2:2A:eGFP mutant fish: showing a swim bladder and regular-sized head; or showing GFP expression in eif2b2^{sa17223/sa17223};β-actin:EIF2B2:2A:eGFP mutant fish. (G–J) TUNEL and DAPI antibody staining of wild-type control; eif2b2^{sa17223/sa17223} mutant; eif2b2^+/+;β-actin:EIF2B2:2A:eGFP wild-type control; and eif2b2^{sa17223/sa17223};β-actin:EIF2B2:2A:eGFP mutant. (K) Quantification of TUNEL+ cells.