FIGURE 6

FIGURE 6

The impact of embryonic low-conductivity stress on cold tolerance of zebrafish. (A) Zebrafish embryos were incubated in low-conductivity water or regular embryo water for indicated times, the mRNA levels of BAG3 and HSPB1 were determined by qRT-PCR (n = 3, two tailed t-test, ^∗∗∗P < 0.001). (B) Three-month-old zebrafish were subjected to cold pressure as in Figure 2A, then survival time was measured. (C) Three-month-old F1 zebrafish of cold tolerant parental fish (low-conductivity-F1) and control F1 zebrafish (control) were exposed to cold pressure as in Figure 2A, then survival time was observed. (D) Three-month-old F1 zebrafish by breeding cold tolerant and control parental fish (low-conductivity-con-F1) and control F1 zebrafish (control) were exposed to cold pressure as in Figure 2A, then survival time was observed.