Impact of embryonic HSP90 inhibition on cold tolerance of zebrafish. (A) Schematic diagram of the cooling time course. The temperature decreased from 28 to 18°C at a rate of 1°C/h, remained at 18°C for 12 h (overnight), then decreased at a rate of 1°C/h until reaching a final temperature of 8°C. (B) Zebrafish from control and radicicol group were exposed to a temperature of 8°C according to the protocol in (A), then survival time and the median lethal time (LT50) were measured. Dotted blue line (bottom) indicates the starting time at 8°C. Broken orange lines indicate the range of survival time of zebrafish of control group. Red box indicates zebrafish with increased survival time (cold tolerant zebrafish). (C,D) Body weight and length of fishes with different survival time for both groups.
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