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Figure 2—figure supplement 1.

ID: ZDB-IMAGE-201223-22
Publication: Chin et al., 2020 - Cohesin mutations are synthetic lethal with stimulation of WNT signaling
All Figures: Figures for Chin et al., 2020

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Figure Caption

Figure 2—figure supplement 1. Cohesin deficiency causes a modest increase in chromosome mis-segregation events in MCF10A cells.

(A) Frequency of aneuploidy in cohesin-deficient MCF10A clones compared to parental MCF10A (WT). (B) Threshold compactness in the nuclei of cohesin-deficient clones compared to wild type MCF10A (R.U. = relative units). n = 5 independent experiments (square symbols), mean ± s.d., one-way ANOVA: ***p≤0.001. (C) Representative images of abnormal interphase nuclei showing chromosome bridges, micronuclei, and lagging chromosomes at anaphase. (D) Quantification of chromosome bridges and micronuclei events in MCF10A cohesin-deficient cells. (E) Quantification of chromosome mis-segregation observed in MCF10A cohesin-deficient clones. At least 100 mitotic cells were examined per condition, n = 3 independent experiments, mean ±s.d., one-way ANOVA **p≤0.01; ***p≤0.0005; ns, not significant.

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