ZFIN Image: Lu et al., 2020, Figure 5

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Figure Caption

Figure 5

ZDW pretreatment attenuated AA-induced defective pronephric ducts. Immunohistochemical staining of the basolateral marker Na⁺/K⁺-ATPase alpha1 subunit (α6F) was performed on transverse sections of the pronephric tubes from 48 hpf zebrafish embryos after exposure to water (no treatment group, (a)), 3 ppm of AA (b), 10 ppm of ZDW (c), and AA combined with ZDW (pretreatment (ZDW; AA group) (d) or cotreatment (ZDW+AA) (e)). The sections were stained with a monoclonal antibody (α6F) and counterstained with hematoxylin. (a′)-(a^″), (b′)-(b^″), (c′)-(c^″), (d′)-(d^″), and (e′)-(e^″) were different positions of the embryos indicated by red lines shown in (a), (b), (c), (d), and (e), respectively.

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