PMA exerted various deformities in zebrafish embryo and larvae (n = 10/group). (A) Morphological images of the development of zebrafish embryo to larval stage. Images were captured from 0.5–6 dpf. Common observed deformities were C (coagulation), CE (cardiac edema), CT (curved tail), LF (lack of tail fins), YB (development of yolk bags. Scale bar = 200 μM at 4× magnification. (B) Percentage of survivability (C) Percentage of hatching rate (D) Determination of heartbeat per minute of on 1 dpf, 2 dpf, and 4 dpf. (E) The data are represented as the means ± S.D. of three independent experiments * p < 0.05, ** p < 0.01, *** p < 0.001 and ns (non-significant). Statistical significance analysis was carried out through a one-way analysis of variance (ANOVA) prism.
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