ZFIN Image: Høgset et al., 2020, Fig. 1

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Figure Caption

Fig. 1 Schematic overview of the confocal Raman spectroscopic imaging applications in zebrafish embryos developed in this study.

a Confocal Raman spectroscopic imaging (cRSI) is a label-free imaging technique that uses molecular vibrations from Raman scattering to generate biomolecular images of the zebrafish embryo. b Characterization and tissue analysis of zebrafish embryos. cRSI can provide three-dimensional biomolecular information from an entire zebrafish embryo or high-resolution imaging of specific tissue regions. c Biomolecular profiling of bacterial infection. cRSI can be used to probe local biomolecular variation in bacterial populations after infection. We demonstrated this approach by using volumetric cRSI to discriminate between wild type and region of difference 1 (ΔRD1) mutant Mycobacterium marinum in a zebrafish infection model. d In vivo time-lapse analysis of wound response. In vivo cRSI can be used to collect Raman spectroscopic images at multiple time points in living embryos and used for time-lapse biomolecular analysis. We demonstrated this concept by following a wound response model for 12 h in living zebrafish embryos.

Acknowledgments

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