ZFIN Image: Loontiens et al., 2020, Fig. 4

Image

Figure Caption

Fig. 4

PHF6 modulates Notch1 expression and its downstream signaling activity. (A) (left) Flow cytometry analysis of control and PHF6 shRNA transduced CD34⁺ thymocytes in OP9-DLL1 cocultures in the presence of IL7, SCF and FLT3L, showing the development of CD4⁺CD8β⁺ DP thymocytes after 21 days of coculture. (right) Bar plot showing the frequency of CD4⁺CD8β⁺ DP thymocytes, generated in the corresponding cultures. Data shows the average of 4 independent experiments and errors bars show SEM. *P < 0.05 (paired t-test) (B) Normalized NOTCH1 and DTX1 expression in Jurkat cells (left) and CB-derived CD34⁺ HPCs (right) following control or PHF6 shRNA transduction as indicated. Data shows the average expression in 3 independent samples and error bars indicate SEM.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol