Fig. 7

Fig. 7

a WB analysis of the level of RRP7A expression and phosphorylation of retinoblastoma protein (p-RB) and CDK1 (p-CDK1^T161) in control and patient HDF cells subjected to serum depleted for 48 h followed by serum addition for 0–24 h. b Quantification of relative levels of p-RB shown in a (data average of n = 4 independent experiments; 0 h: P = 0.537, 6 h: P = 0.105, 12 h: P = 0.016, 16 h: P = 0.023, 20 h: P = 0.005, 24 h: P = 5.035E-07). c Quantification of relative levels of p-CDK1^T161 shown in a (data average of n = 4 independent experiments; 0 h: P = 0.984, 6 h: P = 0.157, 12 h: P = 0.192, 16 h: P = 0.014, 20 h: P = 0.004, 24 h: P = 6.358E-08). d Quantification of relative levels of BrdU incorporation in growth-arrested control and patient HDFs stimulated with serum for 18 h (data average of n = 3 independent experiments; P = 0.001). Data are represented as mean ± SD and significance was determined using an unpaired, two-tailed Student’s t test. *P < 0.05, **P < 0.01, ****P < 0.0001, n.s.: not significant.