Figure 2

Figure 2

Absorption of NLCs in the larval body. (A) TgBAC(cldn15la:GFP)^pd1034 larvae, at 5 days post fertilization (dpf), exposed to NLCs loaded with rhodamine-PE, by the immersion method. The larvae intestine is demarked with a black dotted line. (B) Timeline: fluorescence was monitored at 1, 12 and 24-h post incubation (hpi). Representative images of larvae examined under a fluorescence stereomicroscope at 1 hpi (C), 12 hpi (E) and 24 hpi (G). A region of the mid-intestine of TgBAC(cldn15la:GFP)^pd1034 larvae, demarked by a red dotted line in (C,E,G) was examined under confocal microscope at 1, 12 and 24 hpi (D,F,H, respectively). NLC-R: NLCs labeled with red fluorescent rhodamine-PE. Scale bar 200 μm. The images in A, C–H were edited with ImageJ software v.1.52a (https://imagej.nih.gov/ij/).