ZFIN Image: Abdul Satar et al., 2020, Figure 6

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Figure Caption

Figure 6

Neural tracer application into the median raphe. (a–f) Mid-sagittal visualization of mCherry (magenta)-labelled Kiss1 efferent projection from the ventral habenula (vHb, b) through the fasciculus retroflexus (FR) towards the median raphe (MR, c) in the brain of Tg(kiss1:mCherry) zebrafish without neural tracer implantation (d–f). (g–i) A thin-strip of the nylon membrane-coated with NeuroVue tracer (NeuroVue Maroon, green) was selectively inserted into the MR (h,i). (j–l) Photomicrograph of mid-sagittal visualization of tracer-labelled neural tracks (arrow heads) from the MR (g) towards the vHb (j). Scale bars (a,d) 500 μm; (b,c,e,f,j–l) 20 μm; (g) 200 μm; (h,i) 50 μm.

Acknowledgments

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