Effect of Kiss1 on dopamine levels in different brain regions. (A) Schematic sagittal view of a zebrafish brain illustrating the macro-dissected brain regions that were subjected to analyses of dopamine content levels and the distribution of dopaminergic cell populations (blue-shaded zones). (B–D) Dopamine content levels (ng per mg protein; n = 6 fish/group) in macro-dissected telencephalon (B), preoptic-pretectal (C) and midbrain-hypothalamic (D) regions, 30-min, 1-h, 3-h and 6-h after central administration of either vehicle (control; distilled water) or Kiss1 with doses of 10–12 or 10–9 mol/ fish. Data presented as mean ± SD. *P < 0.05; **P < 0.01 using 2-way ANOVA followed by Tukey’s multiple comparison test.
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