FIGURE 5

FIGURE 5

Wnt/β-catenin signaling is activated during the early wound healing stage in the regenerating brain. (A) Relative expression levels of egfp as a reporter of Wnt/β-catenin signaling activity in the lesioned and unlesioned hemispheres of the telencephalon during the early wound healing and proliferative stages of regeneration. Three hemispheres were pooled for each sample. (B) Relative expression levels of EGFP as a reporter of Wnt/β-catenin signaling activity in the lesioned hemispheres of three independent telencephalon samples used for RNA sequencing during the early wound healing stage. (C) Relative expression levels of the known Wnt target genes lef1 and egr2a in the lesioned hemisphere of the telencephalon during the early wound healing stage. (D,E) Anti-phospho-β-catenin staining of control brain sections. (F,G) Anti-phospho-β-catenin staining of 20-hpl brain sections. The lesioned area is boxed and shown in (H,I). Sections are counterstained for DAPI. Scale bars, 200 μm in (D–G); 25 μm in (H,I). (J) Relative expression levels of egfp as a reporter of Wnt/β-catenin signaling activity in lesioned + IWR-1-treated hemispheres of three independent telencephalon samples collected at 20 hpl. (K) Relative expression levels of the Wnt target genes lef-1 and egr2a in the pooled three lesioned + IWR-1-treated hemispheres at 20 hpl. Statistical significance was evaluated using unpaired t-test. **p < 0.01 and ***p < 0.001. Error bars represent ± standard error of the mean (SEM, n = 3). hpl, hours post-lesion. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.