Figure 8

Figure 8

ELISA of cell conditioned media demonstrates that 24 h treatment with 20 μM quininib analogues increases angiogenic markers in Mel285 cells and OMM2.5 cells. In Mel285 cells, 24 h treatment with 20 μM quininib (Q1) significantly increased the secretion of VEGF-C (A) and bFGF (B) Treatment with 20 μM 1,4-dihydroxy quininib (Q7) significantly increased the secretion of Flt-1 (C) In OMM2.5 cells, 24 h treatment with quininib significantly increased the secretion of Flt-1 (C) and VEGF-A (D) Treatment with 20 μM montelukast or HAMI 3379 had no effect on the secretion of inflammatory markers in either cell line. Conditioned media were collected from three separate experiments and analysed by ELISA (n = 3). All secretions were normalised to total protein content. Statistical analysis was performed by ANOVA with Dunnett’s post hoc multiple comparison test. Error bars are mean + S.E. * p < 0.05, ** p < 0.01, *** p < 0.001.