Figure 6

Figure 6

Quininib and 1,4-dihydroxy quininib reduce colony formation in Mel285 and OMM2.5 cells. Graphs show the percentage survival fraction of clones at 24 (A,C) and 96 h (B,D) post treatment with quininib (Q1), 1,4-dihydroxy quininib (Q7), montelukast (Mon), or HAMI 3379 (HAMI). Images of clones captured by GelCount™ system (Oxford Optronix) after 10 days of culture following treatment with DMSO control or 20 μM quininib (Q1) or 20 μM 1,4-dihydroxy quininib (Q7) for 24 or 96 h. Clones were stained with 0.5% crystal violet before counting. 1500 cells (Mel285) or 9000 cells (OMM2.5) were seeded and treated in duplicate in 6-well plates for each individual experiment and individual experiments were conducted three times (n = 3). Statistical analysis was performed by ANOVA with Dunnett’s post hoc multiple comparison test. Error bars are mean + S.E. *** p < 0.001; **** p < 0.0001.