Figure 10

Figure 10

CysLT₁ antagonists inhibit the growth of UM cell lines in in vivo zebrafish xenograft models. Zebrafish cell line-derived xenograft models were developed using Mel285 and OMM2.5 cell lines. Labelled UM cells were implanted into the perivitelline space (PVS) or vitreous of 48 hpf (hours post-fetilisation) Tg(fli1a:EGFP)^y1 zebrafish embryos and treated with the maximum tolerated dose of test compound, or DMSO control. (A) Treatment with 3 μM quininib (Q1), 10 μM 1,4-dihydroxy quininib (Q7) and 20 μM montelukast (Mon) significantly reduced the growth of Mel285 xenografts in the zebrafish eye (p < 0.0001). (B) Treatment with 10 μM 1,4-dihydroxy quininib and 20 μM montelukast significantly reduced the growth of OMM2.5 xenografts in the zebrafish eye (p = 0.0258 and p = 0.0439, respectively). Relative change in tumour volume was evaluated as the size of the tumours at three days post implantation (3 dpi/5 dpf) relative to the size immediately after implantation (at 0 dpi/2 dpf).