Image
Figure Caption

Figure 2—figure supplement 3.

Overexpression of apln does not cause ectopic EC sprouting.

Confocal projection images of the trunk region of Tg(fli1a:LIFEACT-GFP) embryos at 23 and 48 hpf. apln expression was induced by a single heatshock at 19.5 hpf for 1 hr at 39°C and analyzed at 23 hpf or heatshocked at 42 and 45 hpf for 1 hr at 39°C and analyzed at 48 hpf. Global overexpression of apln does not lead to ectopic vascular sprouting but mispatterning of the string of parachordal lymphangioblasts (arrows).