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Figure 1—figure supplement 3.

ID: ZDB-IMAGE-201021-6
Publication: Helker et al., 2020 - Apelin signaling drives vascular endothelial cells towards a pro-angiogenic state
All Figures: Figures for Helker et al., 2020

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Figure Caption

Figure 1—figure supplement 3. Quantification of angiogenic defects.

Confocal projection images of the trunk region of Tg(fli1a:EGFP) embryos at 30 hpf. Analysis of the offspring of apln ^+/-; apela^+/- (n = 85) and aplnra ^+/-; aplnrb ^+/- (n = 71) fish resulted in different phenotypic categories according to the extent of ISV development. Genotyping of single embryos revealed an additive effect for apln and apela ligands and aplnra and aplnrb receptors. Mild phenotypes correlated with loss of single ligand or receptor genes, while deficiency in apln resulted in stronger phenotypes than deficiency in apela. aplnrb deficiency resulted in stronger phenotypes than deficiency in aplnra. Deficiencies in both ligands or both receptors resulted in more severe phenotypes. Scale bar: 40 µm.

Acknowledgments

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