Image
Figure Caption

Figure 3—figure supplement 2.

Apelin signaling functions cell-autonomously in ECs.

Confocal projection images of the trunk region of Tg(fli1a:EGFP) embryos at 54 hpf. At the blastula stage, cells from Tg(kdrl:HsHRAS-mCherry) embryos were transplanted into host embryos obtained from Tg(fli1a:EGFP) aplnra +/-; aplnrb +/- incrosses. At 54 hpf, the mosaic embryos were imaged and the donor-derived ECs scored for their position. Notably, wild-type ECs transplanted into aplnr deficient embryos completely substituted for the lack of cells in the dorsal part of the vasculature at 54 hpf (Figure 3—figure supplement 1). Scale bar: 30 µm.

Acknowledgments
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