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Figure 3

ID: ZDB-IMAGE-201021-10
Publication: Helker et al., 2020 - Apelin signaling drives vascular endothelial cells towards a pro-angiogenic state
All Figures: Figures for Helker et al., 2020

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Figure 3

Apelin signaling promotes the sprouting behavior of ECs.

(A) Experimental design: At the blastula stage, cells from Tg(kdrl:HsHRAS-mCherry) embryos were transplanted into host embryos obtained from Tg(fli1a:EGFP) aplnra ^+/-; aplnrb ^+/- incrosses. At 24 hpf, the mosaic embryos were imaged and the donor-derived ECs scored for their position. (B, C) 34,5% of wild-type donor-derived ECs in wild-type hosts were found within the ISVs. 80% of wild-type donor-derived ECs in aplnra ^+/-; aplnrb ^-/- hosts were found within the ISVs. Notably, wild-type ECs transplanted into aplnr- deficient embryos completely substituted for the lack of cells in the dorsal part of the vasculature at 54 hpf (Figure 3—figure supplement 2). Scale bars: B, 20 µm.

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