ZFIN Image: Förster et al., 2020, Figure 1.

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Figure Caption

Figure 1. Experimental paradigm for studying location-specific processing in the tectum.

(A) In a typical hunting sequence, the fish detects prey in its peripheral visual field (1), ultimately turns and approaches to bring the prey image into its central binocular field (2). Hypothetically, the retinotectal map might be adapted to this location- and size-specific representation of the prey object. (B) Sketch of the tectum showing previously described cell types and neuropil layers. (C) Schematic for functional imaging setup. (D) On the left: Region of interest (ROI) for imaging tectal cell responses and exemplary expression of nuclear-localized GCaMP6s. On the right: ROI for RGC imaging and expression of GCaMP6s in RGC axons under control of ath5:Gal4. (E) Stimulus protocol. Arrows below stimulus representation indicate object movement, first in nasal, then in temporal direction. See Materials and methods for details. Scale bar in (D): 20 µm.

Acknowledgments

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