Figure 5—figure supplement 3.

Figure 5—figure supplement 3.

Maximum likelihood phylogeny of selected metazoan Fox genes. The multiple sequence alignment consists of 52 sequences aligned over 315 positions (proportion of gaps and undetermined characters: 25.07%). It is generated from OG_36001 (FoxH1), OG_63374 (RBH with OG_36001; orthogroup ID labeled in red), and representative sequences of OG_3972 (FoxD4 as outgroup; third-best hit of OG_36001 in HMM-HMM searches, see Supplementary file 1–Supplementary Table 14) of the original clustering. Selected FoxQ1 proteins were used as outgroup as FoxQ1 resembled the closest relative of FoxH1 proteins in other studies (Yu et al., 2008; Fritzenwanker et al., 2014). Vertebrate and protostomian FoxH1 sequences are decorated with a red and green bar, respectively. Sequences derived from genomic and transcriptomic ORFs are labelled with "|orf_", "|trs_", or "|predict_". All other sequences can be accessed at NCBI with the given identifiers. Branch labels correspond to the results of SH-aLRT (Shimodaira–Hasegawa-like approximate likelihood ratio test, left) and UFBoot (ultrafast bootstrap approximation, right) as implemented in IQ-TREE (Nguyen et al., 2015).