Figure 4—figure supplement 1.

Figure 4—figure supplement 1.

Top: View of a 194 AA alignment of OG_26631 with 36 sequences from 2 chelicerate, 1 myriapod, 2 crustacean, and 23 hexapod species. Center: View of a 165 AA alignment of OG_34551 with 28 sequences from 4 chelicerate, 1 myriapod, 2 crustacean, and 19 hexapod species. Bottom: View of a 155 AA alignment of OG_35928 with 27 sequences from 2 chelicerate, 1 myriapod, and 21 hexapod species. The alignments illustrate the existence of domain-like conservation patterns despite the absence of known protein domains. Short stretches of unaligned sequences were removed for clarity. Colouring of amino acids reflects chemical similarity (UGENE standard colour scheme; Okonechnikov et al., 2012). Bar chart on top displays sequence conservation of alignment columns beneath. Additional HMM-HMM searches suggest that the conserved regions constitute protein domains restricted to arthropods (Supplementary file 1–Supplementary Table 13).