ZFIN Image: Huang et al., 2020, Figure 4

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Figure Caption

Figure 4—figure supplement 1. Characterization of constitutive ITGA2 expression in selected ovarian cancer cell lines.

(A) The levels of ITGA2 and ITGB1 expression in WT versus ITGA2-overexpression (OE) in SKOV3ip, BG1, OVCAR4, and A2780 cell lines were determined by flow cytometry and (B) Western blot. (C) Cell-ECM adhesion assay of WT and ITGA2 overexpressed (OE) ovarian cancer cells. Bar chart summarizes percentage of cell to ECM adhesion efficiency from three independent experiments (***p<0.001, unpaired Student’s t-test). (D) Colony formation assay for WT and OE cells for 7 days. Bar chart represents the mean ± SD of total colony counts. (E) Anchorage-independent soft agar assay for WT and OE cells for 10 days in culture. Bar chart shows the quantification of total spheroid numbers (*p<0.05, unpaired Student’s t-test). Scale bar 200 μm. (F) Anchorage-independent growth for IGROV1 WT and ΔITGA2 cells for 10 days in culture. Bar chart shows quantification of total spheroid numbers and diameter in μm (***p<0.001, unpaired Student’s t-test). Scale bar 100 μm.

Acknowledgments

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