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Fig. EV4

ID: ZDB-IMAGE-201012-104
Publication: Pini et al., 2020 - ALX1-related frontonasal dysplasia results from defective neural crest cell development and migration
All Figures: Figures for Pini et al., 2020

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Fig. EV4 <styled-content toggle='no' style='fixed-case'>CRISPR</styled-content>/Cas9 targeted mutagenesis of <italic>alx1</italic> in zebrafish

Human ALX1 and zebrafish alx1 protein sequences were obtained from Ensembl and aligned using Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) under the default settings. The homeobox DNA‐binding domain is shown in bold, with the amino acid residue mutated in the subjects indicated by an outline. The transactivation domain is shaded in gray. Zebrafish alx1 CRISPR sites #1 and #2 are highlighted in yellow. The red and blue letters visually demarcate the sites.

Schematic diagram shows the effect of the mutant allele resulting from our choice of target site #1. The allele, termed, alx1^uw2016, has a net deletion of 16 nucleotides. Red letters denote the abnormal sequence that results from the frameshift mutation.

Acknowledgments

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