Figure 6—figure supplement 3.

Figure 6—figure supplement 3.

Two maximum likelihood phylogenies of representative bilaterian Slit sequences. Sequences were downloaded from NCBI or extracted from the corresponding Slit orthogroup OG_5717. In subfigure (A), the multiple sequence alignment consists of 19 sequences aligned over 1,800 positions (proportion of gaps and undetermined characters: 24.15%). In (B), a single protein from the placozoon Trichoplax adhaerens was added to the dataset, generating an alignment of 20 sequences over 1,865 positions (proportion of gaps and undetermined characters: 26.73%). Branch labels correspond to the results of SH-aLRT (Shimodaira–Hasegawa-like approximate likelihood ratio test, left) and UFBoot (ultrafast bootstrap approximation, right) as implemented in IQ-TREE (Nguyen et al., 2015). Tree topology and corresponding bootstrap values are compatible with assigning the placozoan sequence to the Slit protein family.