IMAGE

Fig. 2

ID: ZDB-IMAGE-201009-3
Publication: Chakraborty et al., 2020 - Converting lateral scanning into axial focusing to speed up three-dimensional microscopy
All Figures: Figures for Chakraborty et al., 2020

Image

Figure Caption

Fig. 2 Remote focusing using a remote step mirror and a planar mirror inclined by 7.5°.

a PSFs obtained using a remote mirror with three steps. b PSFs generated from discrete lateral scanning steps over a planar, tilted mirror. Green arrows depict the zoomed-in view. c Plot showing the full width at half maximum (FWHM) of the PSFs at different axial focus positions. The FWHM of the PSF calculated in the X-direction (red) and Y-direction (black). d Continuous axial scanning obtained by driving the GSM with a triangular waveform at 100 Hz using a tilted, planar mirror. Scale bar: a–d 10 µm. The scale bar in the zoomed-in image of b is 1 µm

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Light Sci Appl