FIGURE 3

FIGURE 3

vwa1 expression in developing zebrafish embryos. In situ hybridization (ISH) results showing the expression of vwa1 at 19 hpf (A,B), 1 dpf (C,D), 2 dpf (E,F), 3 dpf (G,H) and 4 dpf (I,J) stages in lateral (A,C,E,G,I), dorsal (B,D) and lateral-ventral (F,H,J) views. At 19 hpf and 1 dpf, vwa1 was expressed throughout the body, with expression in the head being higher than that in other parts of the body (black arrows in A,C). At 19 hpf and 1 dpf, vwa1 expression was detected at the medial edge of the pharyngeal arch (black arrows in B,D). At 2 dpf, vwa1 staining in the head began to increase slightly, especially in the mandible (black arrow in E). At 3 dpf and 4 dpf, expression of vwa1 was restricted to the mandible (black arrows in G,I). Staining of vwa1 in the pharyngeal pouch was stronger than that in the pharyngeal arch (black arrows in F–H). vwa1 was also expressed in the epidermis of the brain (D) and in somites (C,E,G,I).