Assessment of the cardiac rhythm of zebrafish larvae treated with food additives. (a) The heartbeat rates of zebrafish larvae (3, 6, and 9 d.p.f.) determined before the exposure to the food additive is the control group (*p < 0.05). (b–d) Heartbeats were recorded from a zebrafish larvae (3, 6, and 9 d.p.f., respectively) treated with varying concentrations of food additives and their comparison with the control group. Cochineal Red additives with 0.2‰ concentration caused an increase in the heartbeat during the early developmental stages of zebrafish larvae. Heart beats per minute are expressed as means ± SD (*p < 0.05, **p < 0.01 and ***p < 0.001).
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