Decreased VAChT expression along the rostral myosepta of 6-day-old larva. (A) Schematic representation of the imaged areas in (B, C). (B, C) Confocal image of a lateral view of larva showing a rostro-caudal gradient of VAChT puncta in MN axons projecting along the dorsal myosepta. Nuclei are labeled by Hoechst (blue). (B′, C′) Zoomed in images of the boxed areas in (B, C). White arrows point toward VAChT positive signals along the myosepta. Scale bars: 50 μm. (D) Quantification of VAChT puncta in the dorsal myosepta. N = 4 larvae (15 rostral axons, 17 caudal axons). 2-tailed non-paired Student’s t test; ****p < 0.0001.
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